Composite

Part:BBa_K3726050:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)


BOH3_LV1 (B)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1533
    Illegal NheI site found at 1003
    Illegal NheI site found at 1026
    Illegal NheI site found at 5008
    Illegal NheI site found at 5236
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1533
    Illegal BglII site found at 6347
    Illegal BglII site found at 6464
    Illegal BglII site found at 8883
    Illegal BamHI site found at 3070
    Illegal BamHI site found at 3409
    Illegal XhoI site found at 4342
    Illegal XhoI site found at 7567
    Illegal XhoI site found at 8410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 18
    Illegal PstI site found at 1438
    Illegal PstI site found at 1686
    Illegal PstI site found at 2987
    Illegal PstI site found at 6002
    Illegal NgoMIV site found at 1177
    Illegal NgoMIV site found at 1277
    Illegal NgoMIV site found at 1583
    Illegal NgoMIV site found at 3665
    Illegal AgeI site found at 594
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 3193
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the trascriptonal unit is flanked by the parts “BBa_K3726104” (5CON1(H)_NS1(mod)-up (PCC 11801)) and “BBa_K3726105 ” (3CON5(H)_NS1(mod)-down (PCC 11801)) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.